abstract：:The objective of this study was to understand the effect of pH and ionic strength of aluminum sulfate on the flocculation of microalgae. It was found that changing pH and ionic strength influenced algal flocculation by changing the zeta potential of cells, which was described by the classical theory of Derjaguin, Land...

journal_title：Applied biochemistry and biotechnology

authors： Cui Y,Yuan W,Cheng J

更新日期：2014-08-01 00:00:00

abstract：:Microalgae have tremendous potential as a renewable feedstock for the production of liquid transportation fuels. In natural waters, the importance of physical associations and biochemical interactions between microalgae and bacteria is generally well appreciated, but the significance of these interactions to algal bio...

journal_title：Applied biochemistry and biotechnology

authors： Bagwell CE,Piskorska M,Soule T,Petelos A,Yeager CM

更新日期：2014-08-01 00:00:00

abstract：:Moth bean (Vigna aconitifolia (Jacq.) Marechal), an important grain-legume crop grown in hot desert regions of Thar, under scorching sun rays, was investigated for heat tolerance at molecular level. In the present study, we constructed a forward suppression subtractive hybridization (SSH) cDNA library of heat tolerant...

journal_title：Applied biochemistry and biotechnology

authors： Gurjar K,Rampuria S,Joshi U,Palit P,Bhatt KV,Mohapatra T,Sharma R

更新日期：2014-08-01 00:00:00

abstract：:Paraoxonase 1 (PON1: EC 3.1.8.1) is a calcium-dependent enzyme associated with high-density lipoproteins (HDLs) and has a protective effect against oxidation of low-density lipoproteins (LDLs) in mammals. PON1 is the best-studied member of a family of enzymes called serum paraoxonases, or PONs, identified in mammals a...

journal_title：Applied biochemistry and biotechnology

authors： Erzengin M,Demir D,Arslan M,Sinan S

更新日期：2014-08-01 00:00:00

abstract：:Anaerobic digestion (AD) process is a well-established method to generate energy from the organic wastes both from the environmental and economical perspectives. The purpose of present study is to evaluate energy production from potato wastes by incorporating cow manure into the process. Firstly, a laboratory pilot of...

journal_title：Applied biochemistry and biotechnology

authors： Sanaei-Moghadam A,Abbaspour-Fard MH,Aghel H,Aghkhani MH,Abedini-Torghabeh J

更新日期：2014-08-01 00:00:00

abstract：:Tradescantia pallida (Wandering jew)-a succulent perennial herb-was screened to be a potent chromium (Cr) accumulator. Its ability to grow under Cr stress was examined by studying biochemical changes and physiological response of the plant in presence of 5-20 mg L(-1) Cr(VI) concentration in hydroponic environment for...

journal_title：Applied biochemistry and biotechnology

authors： Sinha V,Pakshirajan K,Chaturvedi R

更新日期：2014-08-01 00:00:00

abstract：:Non-Systematic Evolution of Ligands by EXponential enrichment (SELEX)selection of aptamers, a novel technology for aptamer selection from libraries of random DNA (or RNA) sequences, involves repetitive steps of partitioning without polymerase chain reaction (PCR) amplification between them. This selection is based on ...

journal_title：Applied biochemistry and biotechnology

authors： Yu X,Yu Y

更新日期：2014-08-01 00:00:00

abstract：:Dual-responsive poly(N,N-diethylacrylamide) (PDEA) hydrogel films with entrapped horseradish peroxidase (HRP) and glucose oxidase (GOD) were successfully prepared on electrode surface with a simple one-step polymerization procedure under mild conditions, designated as PDEA-HRP-GOD. Cyclic voltammetric (CV) response of...

journal_title：Applied biochemistry and biotechnology

authors： Yao H,Lin L,Wang P,Liu H

更新日期：2014-08-01 00:00:00

abstract：:An improved procedure has been developed for efficient somatic embryogenesis in Anethum graveolens. Green friable embryogenic callus was obtained from hypocotyl segments on medium augmented with 2,4-dichlorophenoxyacetic acid (2,4-D). The highest embryogenic callus induction frequency of 87 % was obtained on Murashige...

journal_title：Applied biochemistry and biotechnology

authors： Dhir R,Shekhawat GS,Alam A

更新日期：2014-08-01 00:00:00

abstract：:Gastric cancer is the second most common cause of cancer-related death worldwide. This study was designed to examine the role of CUGBP1 in cell growth via an RNA interference (RNAi) lentivirus system in gastric cancer cells in vitro. The expression of CUGBP1 was much stronger in gastric cancer tissues than that in adj...

journal_title：Applied biochemistry and biotechnology

authors： Wang X,Wang H,Ji F,Zhao S,Fang X

更新日期：2014-07-01 00:00:00

abstract：:A family B DNA polymerase gene from the hyperthermophilic crenarchaeon Ignicoccus hospitalis KIN4/I was highly expressed under the control of T7lac promoter of pET-28ARG in Escherichia coli BL21-CodonPlus(DE3)-RIL cells. The produced I. hospitalis (Iho) DNA polymerase was purified by heat treatment followed by HisTrap...

journal_title：Applied biochemistry and biotechnology

authors： Seo KJ,Cho SS,Ppyun HW,Youn MH,Kim SH,Seo BS,Kwon ST

更新日期：2014-07-01 00:00:00

abstract：:Superoxide dismutase (SOD, EC 1.15.1.1) is an enzyme catalyzing the dismutation of superoxide radical to hydrogen peroxide and dioxygen. To date, four types of SODs--Cu/ZnSOD, MnSOD, FeSOD, and NiSOD--have been identified. In this study, SOD proteins of Brachypodium distachyon (L.) Beauv. were screened by utilization ...

journal_title：Applied biochemistry and biotechnology

authors： Filiz E,Koc I,Ozyigit II

更新日期：2014-07-01 00:00:00

abstract：:In plants, the final step of cysteine formation is catalyzed by O-acetylserine (thiol) lyase (OAS-TL). The purpose of this study was to isolate and characterize an OAS-TL from the tree legume Leucaena leucocephala (leucaena). Leucaena contains a toxic, nonprotein amino acid, mimosine, which is also formed by an OAS-TL...

journal_title：Applied biochemistry and biotechnology

authors： Yafuso JT,Negi VS,Bingham JP,Borthakur D

更新日期：2014-07-01 00:00:00

abstract：:Drinking water distribution systems are composed of various pipe materials and may harbor biofilms even in the continuous presence of disinfectants. Biofilms formation on five pipe materials (copper (Cu), polyethylene (PE), stainless steel (STS), cast iron (CI), and concrete-coated polycarbonate (CP)) within drinking ...

journal_title：Applied biochemistry and biotechnology

authors： Zhu Z,Wu C,Zhong D,Yuan Y,Shan L,Zhang J

更新日期：2014-07-01 00:00:00

abstract：:An extracellular low molecular weight xylanase (Xyn628) from Streptomyces sp. CS628 was isolated from Korean soil sample, produced in wheat bran medium, purified, and biochemically characterized. Xyn628 was purified 4.8-fold with a 33.78 % yield using Sepharose CL-6B column chromatography. The purified xylanase was ~1...

journal_title：Applied biochemistry and biotechnology

authors： Rahman MA,Choi YH,Pradeep GC,Choi YS,Choi EJ,Cho SS,Yoo JC

更新日期：2014-07-01 00:00:00

abstract：:A putative α-amylase gene (accession number, CP000284) of Methylobacillus flagellatus KT ATCC51484 was cloned in Escherichia coli, and its gene product was expressed and characterized. The purified recombinant enzyme (MFAS) displayed a typical amylosucrase (ASase) activity by the demonstration of multiple activities o...

journal_title：Applied biochemistry and biotechnology

authors： Jeong JW,Seo DH,Jung JH,Park JH,Baek NI,Kim MJ,Park CS

更新日期：2014-06-01 00:00:00

abstract：:The present study was carried out to understand the effect of cortisol on caspase expression in the C2C12 and 3 T3-L1 cells under co-culture system. Cells were co-cultured by using transwell inserts with a 0.4-μm porous membrane to separate C2C12 and 3 T3-L1 preadipocyte cells. Each cell type was grown independently o...

journal_title：Applied biochemistry and biotechnology

authors： Muthuraman P

更新日期：2014-06-01 00:00:00

abstract：:The 5' amino-labeled DNA probe complementary to mga gene of Streptococcus pyogenes was immobilized on carboxylated multiwall carbon nanotubes electrode and hybridized with 0.1-100 ng/6 μl single-stranded genomic DNA (ssG-DNA) of S. pyogenes from throat swab of suspected rheumatic heart disease (RHD) patients. Electroc...

journal_title：Applied biochemistry and biotechnology

authors： Singh S,Kaushal A,Khare S,Kumar A

更新日期：2014-05-01 00:00:00

abstract：:1,4-Dioxane, a contaminant increasingly detected in water supplies, is a public health concern because it is classified as a possible human carcinogen. 1,4-Dioxane can be biodegraded by aerobic bacteria via monooxygenase-catalyzed reactions. While these metalloenzymes require trace metals as cofactors in their catalyt...

journal_title：Applied biochemistry and biotechnology

authors： Pornwongthong P,Mulchandani A,Gedalanga PB,Mahendra S

更新日期：2014-05-01 00:00:00

abstract：:Vacuolar processing enzymes (VPEs) have received considerable attention recently, as they exhibit caspase-1-like cleavage activity and regulate the process of PCD. However, knowledge about their detailed characteristics and structures is relatively limited. In this study, a gamma vacuolar processing enzyme gene, MhVPE...

journal_title：Applied biochemistry and biotechnology

authors： Ran K,Yang H,Sun X,Li Q,Jiang Q,Zhang W,Shen W

更新日期：2014-05-01 00:00:00

abstract：:A green synthesis of iron oxide nanoparticles (FeNPs) was developed using Murraya koenigii leaf extract as reducing and stabilizing agent. UV-vis spectra show that the absorption band centred at a wavelength of 277 nm which corresponds to the surface plasmon resonances of synthesized FeNPs. Fourier transform infrared ...

journal_title：Applied biochemistry and biotechnology

authors： Mohanraj S,Kodhaiyolii S,Rengasamy M,Pugalenthi V

更新日期：2014-05-01 00:00:00

abstract：:Hydrogels are attractive biomaterials for three-dimensional cell culture and tissue engineering applications. The preparation of hydrogels using alginate and gelatin provides cross-linked hydrophilic polymers that can swell but do not dissolve in water. In this work, we first reinforced pure alginate by using polyoxye...

journal_title：Applied biochemistry and biotechnology

authors： Aroguz AZ,Baysal K,Adiguzel Z,Baysal BM

更新日期：2014-05-01 00:00:00

abstract：:Pepsin-solubilized collagen (PSC) was extracted from the skin of sea cucumber Holothuria parva and was fractionally characterized. The PSC from H. parva skin consisted of three α1 chains (α1)3, in contrast to calf skin collagen type I with two α1 and one α2 chains (α1)2α2 with approximately 130 kDa each. The maximum t...

journal_title：Applied biochemistry and biotechnology

authors： Adibzadeh N,Aminzadeh S,Jamili S,Karkhane AA,Farrokhi N

更新日期：2014-05-01 00:00:00

abstract：:Biotechnology is consistently seeking improved enzyme stability. Enzymes have great properties, although their marginal stability limits their applications. Among the strategies for improving stability of the enzymes, chemical modification is a simple and effective technique. In the present study, chemical modificatio...

journal_title：Applied biochemistry and biotechnology

authors： Hassani L,Nourozi R

更新日期：2014-04-01 00:00:00

abstract：:A soluble glucoside 3-dehydrogenase (G3DH) was purified from a newly isolated Sphingobacterium faecium ZJF-D6 CCTCC M 2013251. The enzyme was purified to 35.71-fold with a yield of 41.91 % and was estimated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis with a molecular mass of 62 kDa. The sequences of ...

journal_title：Applied biochemistry and biotechnology

authors： Zhang JF,Yang B,Chen W,Chen J

更新日期：2014-04-01 00:00:00

abstract：:The mutant strain designated as ART18, obtained from the wild-type strain Clostridium acetobutylicum PW12 treated by atmospheric and room temperature plasma, showed higher solvent tolerance and butanol production than that of the wild-type strain. The production of butanol was 11.3 ± 0.5 g/L, 31 % higher than that of ...

journal_title：Applied biochemistry and biotechnology

authors： Li HG,Luo W,Wang Q,Yu XB

更新日期：2014-04-01 00:00:00

abstract：:Aspergillus niger CTBU isolated from local decayed bamboo shoot residue was employed to solid-state fermentation (SSF) of bamboo shoot residue to elevate the content of phytosterols. Strain acclimatization was carried out under the fermentation condition using bamboo shoot as substrate for fermentation performance imp...

journal_title：Applied biochemistry and biotechnology

authors： Zheng XX,Chen RS,Shen Y,Yin ZY

更新日期：2014-04-01 00:00:00

abstract：:Protein A of Staphylococcus aureus has been widely used as an affinity ligand for the purification of immunoglobulin. However, the low elution pH and the sensitivity to alkaline condition restricted the large-scale application of antibody purification. To overcome these disadvantages, the B domain was selected and mut...

journal_title：Applied biochemistry and biotechnology

authors： Xia HF,Liang ZD,Wang SL,Wu PQ,Jin XH

更新日期：2014-04-01 00:00:00

abstract：:Microbial oil accumulation via oleaginous fungi has some potential benefits because filamentous fungi can form pellets during cell growth and these pellets are easier to harvest from the culture broth than individual cells. This research studied the effect of various culture conditions on the pelletized cell growth of...

journal_title：Applied biochemistry and biotechnology

authors： Xia C,Wei W,Hu B

更新日期：2014-04-01 00:00:00

abstract：:Search for efficient low-cost substrate/additives are gaining significant impetus in bacterial cellulose (BC) production. Makgeolli sludge (a traditional Korean wine distillery waste) is enriched with organic acid, alcohol, and sugar. Using makgeolli sludge filtrate (MSF) and Hestrin-Schramm (HS) medium (g/l of distil...

journal_title：Applied biochemistry and biotechnology

authors： Hyun JY,Mahanty B,Kim CG

更新日期：2014-04-01 00:00:00

abstract：:Cellulases can be used for biofuel production to decrease the fuel crises in the world. Microorganisms cultured on lignocellulosic wastes can be used for the production of cellulolytic enzymes at large scale. In the current study, cellulolytic enzyme production potential of Aspergillus fumigatus was explored and optim...

journal_title：Applied biochemistry and biotechnology

authors： Mehboob N,Asad MJ,Asgher M,Gulfraz M,Mukhtar T,Mahmood RT

更新日期：2014-04-01 00:00:00

abstract：:The objective of present investigation was to study the effect of gut microbiota alteration by oral administration of targeted delivery of pH sensitive cefdinir microspheres to high-fructose-fed (HFD) rats. Rats were fed with a high-fructose diet with or without cefdinir microsphere administration for 30 days. The fec...

journal_title：Applied biochemistry and biotechnology

authors： Jena PK,Singh S,Prajapati B,Nareshkumar G,Mehta T,Seshadri S

更新日期：2014-04-01 00:00:00

abstract：:The two six-coordinate Pt(IV) complexes, containing bidentate nitrogen donor/methyl ligands with general formula [Pt(X)2Me2((t)bu2bpy)], where (t)bu2bpy = 4,4'-ditert-butyl-2,2'-bipyridine and X = Cl (C1) or Br (C2), serving as the leaving groups were synthesized for evaluation of their anticancer activities and DNA b...

journal_title：Applied biochemistry and biotechnology

authors： Pouryasin Z,Yousefi R,Nabavizadeh SM,Rashidi M,Hamidizadeh P,Alavianmehr MM,Moosavi-Movahedi AA

更新日期：2014-03-01 00:00:00

abstract：:Jonesia denitrificans BN-13 produces six xylanases: Xyl1, Xyl2, Xyl3, Xyl4, Xyl5, and Xyl6; the Xyl4 was purified and characterized after two consecutive purification steps using ultrafiltration and anion exchange chromatography. The xylanase-specific activity was found to be 77 unit (U)/mg. The molecular weight of th...

journal_title：Applied biochemistry and biotechnology

authors： Boucherba N,Gagaoua M,Copinet E,Bettache A,Duchiron F,Benallaoua S

更新日期：2014-03-01 00:00:00

abstract：:Cross-linked enzyme aggregates (CLEAs) are a versatile and effective method for enzyme immobilization, which is exquisitely simple and amenable to rapid optimization. In this study, nature egg white, which is low cost, easily available, and nontoxic, was used as protein feeder to replace traditional protein feeder (bo...

journal_title：Applied biochemistry and biotechnology

authors： Jiang Y,Wang Q,He Y,Zhou L,Gao J

更新日期：2014-03-01 00:00:00

abstract：:Biosurfactants are of considerable interest due to their biodegradability, low degree of toxicity, and diverse applications. However, the high production costs involved in the acquisition of biosurfactants underscore the need for optimization of the production process to enable viable application on an industrial scal...

journal_title：Applied biochemistry and biotechnology

authors： Campos JM,Stamford TL,Sarubbo LA

更新日期：2014-03-01 00:00:00

abstract：:An aspartic protease gene from Pleurotus ostreatus (Po-Asp) had been cloned based on the 3' portion of cDNA in our previous work. The Po-Asp cDNA contained 1,324 nucleotides with an open reading frame (ORF) of 1,212 bp encoding 403 amino acid residues. The putative amino acid sequence included a signal peptide, an act...

journal_title：Applied biochemistry and biotechnology

authors： Yin C,Zheng L,Chen L,Tan Q,Shang X,Ma A

更新日期：2014-02-01 00:00:00

abstract：:Fluorescent proteins from marine organisms represent potential candidates for biosensor development. In this paper, we described the isolation of a native green fluorescent protein from Anemonia sulcata and the cloning and purification of its equivalent as a recombinant protein in Escherichia coli. Furthermore, the sp...

journal_title：Applied biochemistry and biotechnology

authors： Masullo T,Puccio R,Di Pierro M,Tagliavia M,Censi P,Vetri V,Militello V,Cuttitta A,Colombo P

更新日期：2014-02-01 00:00:00

abstract：:An efficient regeneration system was established for an ethnomedicinal shrub Rhinacanthus nasutus from root-derived callus organogenesis. The root segments were cultured on MS medium supplemented with various concentrations of Kn (1.0-4.0 μM) alone or in combination with IBA (0.2-0.6 μM) or 2, 4-D (0.5-1.5 μM). The op...

journal_title：Applied biochemistry and biotechnology

authors： Cheruvathur MK,Thomas TD

更新日期：2014-02-01 00:00:00

abstract：:A simple, sensitive aptamer-based biosensor for the detection of phenylalanine is developed using the electrochemical transduction method. For this proposed aptasensor, a 5-thiol-terminated aptamer is covalently attached onto a gold electrode. At the first time, the electrode was evaluated as an electrochemical aptase...

journal_title：Applied biochemistry and biotechnology

authors： Omidinia E,Shadjou N,Hasanzadeh M

更新日期：2014-02-01 00:00:00